8P6S

Arabidopsis thaliana DGD2 in complex with UDP-galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	44.032, 44.032, 368.478
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.290 - 2.100
R-factor	0.2061
Rwork	0.203
R-free	0.25658
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	2.211
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.290	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	22546	1576
<I/σ(I)>	20.5
Completeness [%]	99.1
Redundancy	58
CC(1/2)	1.000	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M bicine/Trizma base pH 8.5, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.02M 1,3-Propanediol, 0.02M 1,4-Butanediol, 0.02M 1,6-Hexanediol, 0.02M 1-Butanol, 0.02M 2-Propanol