8P6H

Bovine naive ultralong antibody AbBLV5B8* collected at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	161.160, 49.045, 60.381
Unit cell angles	90.00, 90.87, 90.00

Refinement procedure

Resolution	80.570 - 1.460
R-factor	0.22379
Rwork	0.222
R-free	0.26109
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.528
Data reduction software	xia2 (0.6.374-gccd9eb9a-dials-2.1)
Data scaling software	pointless (1.11.21)
Phasing software	PHASER (5.8.0405)
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.570	1.490
High resolution limit [Å]	1.460	1.460
Rmerge	0.070	1.825
Rmeas	0.076	2.019
Rpim	0.029	0.847
Total number of observations	539903	23229
Number of reflections	82095	4094
<I/σ(I)>	12	0.4
Completeness [%]	100.0
Redundancy	6.6	5.7
CC(1/2)	0.999	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	10% w/v PEG 20000, 20% v/v PEG MME 550, 0.02M Sodium L-glutamate, 0.02M DL-Alanine, 0.02M Glycine, 0.02M DL-Lysine HCl, 0.02M DL-Serine, 0.1 M MOPS/HEPES-Na