8P5Q

Structure of an ALOG domain from Arabidopsis thaliana in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.873
Spacegroup name	P 43 2 2
Unit cell lengths	79.798, 79.798, 127.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.690 - 2.140
R-factor	0.2156
Rwork	0.214
R-free	0.25190
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.537
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.570	2.200
High resolution limit [Å]	2.140	2.140
Rmeas	0.040
Number of reflections	43011	3138
<I/σ(I)>	21.7
Completeness [%]	99.8	99.1
Redundancy	7	7
CC(1/2)	1.000	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	1.6M Ammonium sulfate, 0.1 M Hepes, 0.1M NaCl