8P4W

Lactobacillus plantarum LpdD mutant - H35A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-04-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	58.970, 58.970, 83.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.146 - 1.780
R-factor	0.1951
Rwork	0.194
R-free	0.21970
Structure solution method	MIR
RMSD bond length	0.006
RMSD bond angle	0.749
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.146	1.760
High resolution limit [Å]	1.730	1.730
Number of reflections	15850	748
<I/σ(I)>	13.4
Completeness [%]	100.0
Redundancy	17
CC(1/2)	1.000	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M Sodium Acetate pH 4.6, 2.0M Ammonium Sulphate