8P3K

Fusion hSlp2-a_Rab27A non-covalent complex with compound IMP-2505

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	60.896, 76.536, 117.795
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.090 - 2.580
R-factor	0.2418
Rwork	0.238
R-free	0.31520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.142
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.090	2.672
High resolution limit [Å]	2.580	2.580
Number of reflections	17938	1735
<I/σ(I)>	4.17
Completeness [%]	98.8
Redundancy	6.6
CC(1/2)	0.959	0.216

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M NH4SO4, 0.1 M MES, pH 6.0, 15% PEG 4K