8P2T

Bovine naive ultralong antibody AbD08* collected at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	65.030, 70.860, 96.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.740 - 1.450
R-factor	0.15057
Rwork	0.148
R-free	0.20555
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.639
Data reduction software	xia2 (0.6.374-gccd9eb9a-dials-2.1)
Data scaling software	pointless (1.11.21)
Phasing software	PHASER (5.8.0405)
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.740	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.070	2.604
Rmeas	0.076	2.868
Rpim	0.030	1.189
Total number of observations	526647	21922
Number of reflections	79276	3871
<I/σ(I)>	10.4	0.5
Completeness [%]	100.0
Redundancy	6.6	5.7
CC(1/2)	0.999	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.03M Sodium Fluoride, 0.03M Sodium Bromide, 0.03M Sodium Iodide, 0.1 M MOPS/HEPES-Na