8P2O

Dimeric mutant S103R of the BTB domain of ZBTB8A from Xenopus laevis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 61
Unit cell lengths	87.918, 87.918, 97.795
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.480 - 1.850
R-factor	0.1703
Rwork	0.169
R-free	0.18810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.741
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	1.890
High resolution limit [Å]	1.850	1.850
Rpim	0.044	0.432
Number of reflections	36642	2244
<I/σ(I)>	9.6	1.4
Completeness [%]	100.0	100
Redundancy	20.9	20
CC(1/2)	0.995	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	PEG8000, Na Citrate