8P26
Crystal structure of Arabidopsis thaliana PAXX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-20 |
| Detector | DECTRIS EIGER2 XE 9M |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 107.030, 152.419, 187.087 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 152.420 - 3.600 |
| R-factor | 0.2499 |
| Rwork | 0.248 |
| R-free | 0.28590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.906 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 187.360 | 3.760 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.242 | 1.401 |
| Number of reflections | 36248 | 4339 |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 14.2 |
| CC(1/2) | 0.997 | 0.889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 20%(w/v) PEG550MME, 100 mM NaCl, 100 mM Bicine pH9.0 |
