8P1Y
Arabidopsis thaliana mutated variant C244S of seryl-tRNA synthetase
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 97.385, 46.154, 107.605 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 19.357 - 2.600 |
| Rwork | 0.208 |
| R-free | 0.26577 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.568 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.357 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.074 | 0.574 |
| Number of reflections | 14883 | 1491 |
| <I/σ(I)> | 9.42 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | magnesium chloride, TRIS pH 7, PEG 4000 |
