8P1R
Bifidobacterium asteroides alpha-L-fucosidase (TT1819) catalytic mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 87.684, 136.252, 160.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.074 - 1.659 |
| Rwork | 0.160 |
| R-free | 0.18750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.608 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.074 | 1.690 |
| High resolution limit [Å] | 1.659 | 1.660 |
| Rmerge | 0.204 | 0.804 |
| Rpim | 0.044 | 0.304 |
| Number of reflections | 224526 | 10641 |
| <I/σ(I)> | 11.1 | 1.9 |
| Completeness [%] | 99.1 | |
| Redundancy | 21.1 | |
| CC(1/2) | 0.997 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium acetate pH 4.6, 8% (w/v) PEG 4000 |
