8P1C

Lysozyme structure solved from serial crystallography data collected at 1 kHz with JUNGFRAU detector at MAXIV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	293
Detector technology	PIXEL
Collection date	2021-12-02
Detector	PSI JUNGFRAU 4M
Wavelength(s)	0.826569
Spacegroup name	P 43 21 2
Unit cell lengths	79.060, 79.060, 38.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.580
R-factor	0.1631
Rwork	0.161
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.953
Data reduction software	CrystFEL (0.10.0)
Data scaling software	CrystFEL (0.10.0)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.900	1.640
High resolution limit [Å]	1.580	1.580
Number of reflections	31810	3358
<I/σ(I)>	9.3	0.7
Completeness [%]	100.0	100
Redundancy	504.2
CC(1/2)	0.996	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	3	293	22% NaCl, 6.4% polyethylene glycol 6000, 80 mM sodium acetate, pH 3.0