8P1A

Lysozyme structure solved from serial crystallography data collected at 2 kHz for 5 seconds with JUNGFRAU detector at MAXIV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	293
Detector technology	PIXEL
Collection date	2021-12-05
Detector	PSI JUNGFRAU 4M
Wavelength(s)	1.12716
Spacegroup name	P 43 21 2
Unit cell lengths	78.300, 78.300, 37.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.940 - 2.050
R-factor	0.1752
Rwork	0.172
R-free	0.22570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.780
Data reduction software	CrystFEL (0.10.0)
Data scaling software	CrystFEL (0.10.0)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.150	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	14178	1373
<I/σ(I)>	5.4	1.7
Completeness [%]	99.9	99.7
Redundancy	18.3	12.1
CC(1/2)	0.915	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	3	293	22% NaCl, 6.4% polyethylene glycol 6000, 80 mM sodium acetate, pH 3.0