8P19
USP28 USP domain apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-24 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.873 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 106.634, 106.634, 329.857 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.970 - 2.450 |
| R-factor | 0.1951 |
| Rwork | 0.194 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h4i |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.477 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.970 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.177 | 4.113 |
| Rmeas | 0.182 | 4.221 |
| Rpim | 0.041 | 0.942 |
| Total number of observations | 60001 | |
| Number of reflections | 26861 | 3008 |
| <I/σ(I)> | 13.7 | 0.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 19.3 | 19.9 |
| CC(1/2) | 0.999 | 0.369 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05M NaCl 0.1M Li2SO4 0.1M MES pH 6.4 14% PEG4000 |
