8P0Y
The crystal structure of the C-terminal domain of Mengla nucleoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 |
| Unit cell lengths | 177.182, 177.182, 90.953 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.900 - 4.120 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.822 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (dev_4788) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 4.500 |
| High resolution limit [Å] | 4.120 | 4.120 |
| Rmerge | 0.284 | 1.821 |
| Number of reflections | 12572 | 660 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 83.8 | |
| Redundancy | 5 | |
| CC(1/2) | 0.989 | 0.449 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M NaCl, 0.1M HEPES pH7, 1.6M Ammonium Sulfate |
