8P0P

Crystal structure of AaNGT complexed to UDP-2F-Glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	47.068, 114.005, 258.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	129.000 - 2.730
R-factor	0.19883
Rwork	0.197
R-free	0.24517
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.397
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	258.010	2.880
High resolution limit [Å]	2.730	2.730
Number of reflections	38131	5436
<I/σ(I)>	8.7
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.998	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	291	calcium acetate, sodium acetate, PEG 4000