8OZT
Crystal Structure of Fucosidase B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 167.457, 306.646, 169.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 153.320 - 2.510 |
| R-factor | 0.17084 |
| Rwork | 0.169 |
| R-free | 0.19979 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.726 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 153.320 | 2.640 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 0.271 | 1.168 |
| Rmeas | 0.286 | 1.230 |
| Rpim | 0.090 | 0.380 |
| Total number of observations | 1512871 | 224661 |
| Number of reflections | 148303 | 21532 |
| <I/σ(I)> | 7.9 | 2.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 10.2 | 10.4 |
| CC(1/2) | 0.990 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 3% PEG 8000, 26% MPD, 7% taximate pH5 |
