8OZO
Populus tremula stable protein 1 with N-terminal binding peptide extension
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-26 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.873128 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.839, 94.458, 167.955 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 48.160 - 2.400 |
| R-factor | 0.1846 |
| Rwork | 0.182 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.864 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.160 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.096 | |
| Rmeas | 0.117 | |
| Number of reflections | 118409 | 5849 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.998 | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.2M Potassium sodium tartrate tetrahydrate, 20% PEG 3350 |
