8OZ7

Abortive infection DNA polymerase AbiA from Lactococcus lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	84.090, 86.100, 117.010
Unit cell angles	99.32, 90.18, 90.27

Refinement procedure

Resolution	38.490 - 2.750
R-factor	0.2207
Rwork	0.220
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.490	2.920
High resolution limit [Å]	2.750	2.750
Number of reflections	80248	12735
<I/σ(I)>	9.03
Completeness [%]	95.2	93.3
Redundancy	3.65	3.57
CC(1/2)	0.996	0.561

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M sodium potassium tartrate tetrahydrate, 0.1 M Bis-Tris (pH 6.0), 9% PEG 10 000