8OZ4

Populus tremula stable protein 1 with an alternate crystal lattice

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976253
Spacegroup name	I 4 2 2
Unit cell lengths	89.573, 89.573, 186.746
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.790 - 3.100
R-factor	0.2234
Rwork	0.220
R-free	0.28790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.185
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.760	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.138
Number of reflections	7263	1289
<I/σ(I)>	14.6
Completeness [%]	99.8
Redundancy	18.6
CC(1/2)	0.995	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	0.1M HEPES pH 7.5, 0.2N NaCl, 20% PEG 3350