8OYW

De novo designed rhomboid protease-like fold RPF_9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	39.720, 66.680, 73.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 1.500
R-factor	0.1984
Rwork	0.198
R-free	0.21630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.670
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	1.554
High resolution limit [Å]	1.500	1.500
Rmerge	0.081	1.196
Number of reflections	32093	3164
<I/σ(I)>	11.8	1.45
Completeness [%]	99.6	99.72
Redundancy	8.1	7.5
CC(1/2)	0.998	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	277.15	0.1 M HEPES pH 7.8, 0.15 M sodum citrate tribasic dihydrate, 25% v/v PEG smear low