8OYV
De novo designed Claudin fold CLF_4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.560, 50.770, 54.930 |
| Unit cell angles | 107.61, 90.49, 90.06 |
Refinement procedure
| Resolution | 52.350 - 2.790 |
| R-factor | 0.2744 |
| Rwork | 0.273 |
| R-free | 0.31760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.477 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.350 | 2.895 |
| High resolution limit [Å] | 2.790 | 2.795 |
| Rmerge | 0.107 | 1.588 |
| Number of reflections | 8007 | 858 |
| <I/σ(I)> | 6.34 | 1.42 |
| Completeness [%] | 88.0 | 95.12 |
| Redundancy | 2.5 | 2.7 |
| CC(1/2) | 0.990 | 0.437 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277.15 | 0.1 M sodium citrate pH 5, 0.1 M Sodium/Potassium phosphate pH 5.5, 0.1M Rubidium Chloride, 25% v/v PEG smear medium |
