8OYF
Crystal structure of ASBTNM in lipidic cubic phase without substrate bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.479, 80.609, 86.453 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.960 - 2.100 |
R-factor | 0.2129 |
Rwork | 0.211 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.528 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.000 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.136 | 0.854 |
Rmeas | 0.147 | 0.920 |
Rpim | 0.054 | 0.332 |
Number of reflections | 20744 | 14741 |
<I/σ(I)> | 6.68 | 1.98 |
Completeness [%] | 99.4 | 99.3 |
Redundancy | 7.1 | 7.3 |
CC(1/2) | 0.957 | 0.658 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | 0.1M Sodium Chloride, 0.1M Hepes pH 7.0, 30% PEG 300 |