8OY1
Structure of the human Guanine Nucleotide-Binding Protein G(K) Subunit Alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 113.065, 113.065, 68.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.700 - 3.340 |
| R-factor | 0.2082 |
| Rwork | 0.205 |
| R-free | 0.28010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4746) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.700 | 3.520 |
| High resolution limit [Å] | 3.340 | 3.342 |
| Number of reflections | 5841 | 292 |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 94.9 | 79 |
| Redundancy | 18.8 | |
| CC(1/2) | 0.999 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | 0.1 M sodium MES at pH 6.5 containing 20% PEG 8000 and 0.2 M ammonium sulfate |
