8OXR

Structure of the N-terminal didomain d1_d2 of the Thrombospondin type-1 domain-containing 7A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-04
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.9762
Spacegroup name	C 2 2 21
Unit cell lengths	67.843, 108.714, 53.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.555 - 2.300
Rwork	0.227
R-free	0.29160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.600
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.560	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	9135	855
<I/σ(I)>	10.8	1
Completeness [%]	99.8
Redundancy	13.2
CC(1/2)	1.000	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M sodium chloride, 0.1 M bis-tris, 1.5 M ammonium sulphate, pH 6.5