8OXJ

crystal structure of powdery mildews Blumeria graminis f. sp. hordei AVRA22

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	48.104, 60.763, 66.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.810 - 1.640
R-factor	0.2215
Rwork	0.219
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.639
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.810	1.740
High resolution limit [Å]	1.640	1.650
Number of reflections	24155	7285
<I/σ(I)>	7.51
Completeness [%]	99.1
Redundancy	3.1
CC(1/2)	0.997	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	1.0 M Succinic acid pH 7.0, 0.1 M HEPES pH 7.0, 1% w/v Polyethylene glycol monomethyl ether 2,000