8OWC

Crystal structure of Hen Egg White Lysozyme co-crystallized with 10 mM TbXo4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.648
Spacegroup name	P 43 21 2
Unit cell lengths	77.474, 77.474, 37.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.750 - 1.700
R-factor	0.1771
Rwork	0.175
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.694
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.490	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.097	0.336
Rpim	0.026	0.073
Number of reflections	13059	1848
<I/σ(I)>	22.8	9
Completeness [%]	99.7	98.8
Redundancy	24	23.5
CC(1/2)	0.998	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	650 mM sodium chloride, 100mM sodium acetate pH=4.6. Drops were set by mixing one volume of protein solution at 20mg/ml with one volume of 10mM TbXo4 and with one volume of the well solution.