8OVT

Crystal structure of the ligand-free YeGT glycosyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	156.854, 156.854, 154.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.060 - 2.300
R-factor	0.2063
Rwork	0.204
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.419
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.520
High resolution limit [Å]	2.300	2.300
Rmerge	0.188	2.598
Rpim	0.045	0.580
Number of reflections	57009	2860
<I/σ(I)>	17.1	1.8
Completeness [%]	96.4
Redundancy	38.6
CC(1/2)	1.000	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10 mM Tri-sodium citrate, 16% PEG 6000.