8OVS
Crystal structure of YeGT glycosyltransferase with bound UDP
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.130, 114.546, 69.376 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 47.130 - 1.123 |
| Rwork | 0.153 |
| R-free | 0.16850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.196 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.130 | 1.220 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.132 | 2.038 |
| Rpim | 0.023 | 0.479 |
| Number of reflections | 213705 | 10686 |
| <I/σ(I)> | 18 | 1.7 |
| Completeness [%] | 92.7 | |
| Redundancy | 30.3 | 18.4 |
| CC(1/2) | 0.999 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100mM HEPES pH 7.5, 500mM sodium acetate, 50mM cadmium sulfate |
