8OVQ
Crystal structure of Geodia cydonium sponge adhesion molecule long form (SAML).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.080, 41.160, 77.801 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 38.931 - 1.610 |
| Rwork | 0.151 |
| R-free | 0.20270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.554 |
| Data reduction software | XDS (BUILT=20220110) |
| Data scaling software | XDS (0.7.9) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.800 | 1.640 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.067 | 0.740 |
| Rmeas | 0.088 | |
| Rpim | 0.056 | |
| Number of reflections | 27255 | 1358 |
| <I/σ(I)> | 9.3 | 1.5 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 4.1 | 3.7 |
| CC(1/2) | 0.997 | 0.576 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | PEG4000, 0.2M ammonium sulfate |
