8OV8

Crystal structure of Ene-reductase 1 from black poplar mushroom in complex to NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999869
Spacegroup name	P 21 21 21
Unit cell lengths	83.420, 99.729, 103.099
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.790 - 1.900
R-factor	0.171
Rwork	0.169
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.905
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_4788)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.790	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	67607	67237
<I/σ(I)>	8.1
Completeness [%]	99.0
Redundancy	6.5
CC(1/2)	0.996	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1 M Bicine pH 9.0, 1.6 M Ammonium sulfate