8OV0
MUC5AC CysD7 amino acids 3518-3626
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-23 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.34050 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.659, 22.921, 66.231 |
| Unit cell angles | 90.00, 94.91, 90.00 |
Refinement procedure
| Resolution | 22.000 - 1.700 |
| R-factor | 0.1825 |
| Rwork | 0.179 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.167 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.028 | |
| Rpim | 0.028 | |
| Number of reflections | 10154 | 1036 |
| <I/σ(I)> | 15.3 | |
| Completeness [%] | 97.3 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.998 | 0.975 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 150 mM ammonium chloride 100 mM sodium acetate pH 5.1 32% PEG 3350 5% ethylene glycol |
