8OUP
Structural characterization of the hexa-coordinated globin from Spisula solidissima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93400 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.219, 50.684, 74.165 |
| Unit cell angles | 75.65, 88.09, 85.30 |
Refinement procedure
| Resolution | 49.010 - 1.700 |
| R-factor | 0.1522 |
| Rwork | 0.150 |
| R-free | 0.19685 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.723 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.010 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.036 | 0.120 |
| Number of reflections | 70881 | 10388 |
| <I/σ(I)> | 24.4 | 11.9 |
| Completeness [%] | 95.0 | 94.8 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 2.5 M ammonium sulfate, and 50 mM sodium acetate |
