8OUN
Arf GTPase from the asgard Gerdarchaea : GerdArfR1 bound to GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980097 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.290, 59.930, 72.350 |
| Unit cell angles | 90.00, 102.09, 90.00 |
Refinement procedure
| Resolution | 45.730 - 1.653 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.24020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (20-OCT-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.730 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmeas | 0.070 | 0.730 |
| Number of reflections | 39833 | 2142 |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 91.8 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.999 | 0.734 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | 18% PEG 8000, 0.2 M Calcium acetate, 0.1M Sodium cacodylate pH 6.5 |
