8OUL

Arf GTPase from the asgard Hodarchaea : HodArfR1 bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 43 21 2
Unit cell lengths	75.693, 75.693, 189.306
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.470 - 3.100
R-factor	0.239
Rwork	0.236
R-free	0.28930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (20-OCT-2021))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.300	3.290
High resolution limit [Å]	3.100	3.100
Rmeas	0.048	0.269
Number of reflections	10627	1678
<I/σ(I)>	6.2	1.1
Completeness [%]	99.9	99.9
Redundancy	18.3
CC(1/2)	0.994	0.063

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	30% PEG 4000, 0.1M Tris-HCl pH 8.5, 0.2M Lithium sulfate