8OUA

Cereblon isoform 4 in complex with novel Benzamide-Type Cereblon Binder 11f

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-19
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	56.422, 59.472, 88.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.300 - 1.850
Rwork	0.184
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.816
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	25046	3258
<I/σ(I)>	11.62
Completeness [%]	96.4
Redundancy	12
CC(1/2)	0.998	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	0.5 M (NH4)H2PO4