8OU6

Cereblon isoform 4 in complex with novel Benzamide-Type Cereblon Binder 11c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-19
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	56.989, 59.126, 87.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.120 - 1.840
Rwork	0.184
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.352
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.120	1.910
High resolution limit [Å]	1.840	1.840
Number of reflections	26237	4157
<I/σ(I)>	16.62
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.999	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	0.5 M (NH4)H2PO4