8OU5

Cereblon isoform 4 in complex with novel Benzamide-Type Cereblon Binder 11b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-17
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	56.839, 59.572, 88.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.416 - 2.300
Rwork	0.204
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.958
Data reduction software	XDS
Data scaling software	XDS
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0405)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.420	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	13895	2193
<I/σ(I)>	10.48
Completeness [%]	100.0
Redundancy	12.9
CC(1/2)	0.997	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	0.5 M (NH4)H2PO4