8OU1

Crystal structure of the SPOC domain of mouse SPOCD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-20
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	54.520, 56.624, 97.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.600 - 1.700
R-factor	0.2031
Rwork	0.201
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.037
Data reduction software	XDS (March 15, 2019 (built 20191211))
Data scaling software	pointless (1.11.21)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.520	54.520	1.790
High resolution limit [Å]	1.700	5.380	1.700
Rmeas	0.062	0.030	0.513
Rpim	0.028	0.014	0.266
Number of reflections	33752	1205	4799
<I/σ(I)>	13.2		2.1
Completeness [%]	99.2	99.6	98.7
Redundancy	4.7	4.5	3.4
CC(1/2)	0.999	0.999	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	1.6M Ammonium citrate tribasic, pH 7.5