8OSP

GCN5-related N-Acetyltransferase from Lactobacillus curiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-03-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000021
Spacegroup name	P 1 21 1
Unit cell lengths	60.680, 37.080, 83.650
Unit cell angles	90.00, 97.67, 90.00

Refinement procedure

Resolution	41.450 - 1.950
R-factor	0.1846
Rwork	0.183
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.678
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.450	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.150	0.960
Rmeas	0.163
Number of reflections	26388	2286
<I/σ(I)>	10.07	2.02
Completeness [%]	96.4
Redundancy	11.54
CC(1/2)	0.996	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	1 M Potassium sodium tartrate, 0.1 M MES sodium hydroxide pH 6.0