8OSC
Structure of Homo sapiens 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 (DNPH1) bound to deoxyuridine 5'- monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-08 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.354, 38.772, 66.195 |
| Unit cell angles | 90.00, 120.29, 90.00 |
Refinement procedure
| Resolution | 33.380 - 1.420 |
| R-factor | 0.1979 |
| Rwork | 0.197 |
| R-free | 0.21040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.941 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.380 | 1.440 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.058 | 0.814 |
| Rmeas | 0.060 | 0.855 |
| Rpim | 0.015 | 0.254 |
| Number of reflections | 44559 | 2097 |
| <I/σ(I)> | 24.7 | 4 |
| Completeness [%] | 99.7 | 94.9 |
| Redundancy | 15.4 | 10.9 |
| CC(1/2) | 0.999 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293.15 | [40 mM sodium propionate, 20 mM MES and 40 mM BIS-TRIS propane] pH 4.0 and 20% PEG 1500 |
