8OSB

TWIST1-TCF4-ALX4 complex on specific DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8856
Spacegroup name	P 31 2 1
Unit cell lengths	60.589, 60.589, 237.289
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.000 - 2.900
R-factor	0.22251
Rwork	0.220
R-free	0.26987
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.965
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.990	2.980
High resolution limit [Å]	2.820	2.820
Rmerge	0.212	4.877
Rmeas	0.223	5.126
Rpim	0.068	1.546
Total number of observations	132310	16496
Number of reflections	12686	1581
<I/σ(I)>	8	0.5
Completeness [%]	97.9
Redundancy	10.4	10.4
CC(1/2)	0.995	0.149

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	Sodium Cacodylate buffer (pH 7.5), 100 mM magnesium acetate, 18 % Glycerol, 20 % 2-Methyl-2,4-pentanediol, 6-7% PEG (Mw 8000)