8OS9

Structure of Homo sapiens 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 (DNPH1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-22
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	57.491, 64.517, 62.939
Unit cell angles	90.00, 90.03, 90.00

Refinement procedure

Resolution	45.050 - 1.700
R-factor	0.2047
Rwork	0.203
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.989
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.930	1.729
High resolution limit [Å]	1.700	1.700
Rmerge	0.044	0.609
Rmeas	0.048	0.664
Rpim	0.018	0.261
Number of reflections	45873	2455
<I/σ(I)>	18.3	2.2
Completeness [%]	90.4	99.5
Redundancy	6.8	6.2
CC(1/2)	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293.15	[40 mM sodium propionate, 20 mM MES and 40 mM BIS-TRIS propane] pH 4.0 and 20% PEG 1500