8OS9
Structure of Homo sapiens 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 (DNPH1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-22 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.491, 64.517, 62.939 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 45.050 - 1.700 |
| R-factor | 0.2047 |
| Rwork | 0.203 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.989 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.930 | 1.729 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.044 | 0.609 |
| Rmeas | 0.048 | 0.664 |
| Rpim | 0.018 | 0.261 |
| Number of reflections | 45873 | 2455 |
| <I/σ(I)> | 18.3 | 2.2 |
| Completeness [%] | 90.4 | 99.5 |
| Redundancy | 6.8 | 6.2 |
| CC(1/2) | 0.999 | 0.853 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293.15 | [40 mM sodium propionate, 20 mM MES and 40 mM BIS-TRIS propane] pH 4.0 and 20% PEG 1500 |
