8OS6
Structure of a GFRA1/GDNF LICAM complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.140, 170.044, 130.791 |
| Unit cell angles | 90.00, 96.17, 90.00 |
Refinement procedure
| Resolution | 90.450 - 2.660 |
| R-factor | 0.2456 |
| Rwork | 0.244 |
| R-free | 0.28280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fub |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.469 |
| Data reduction software | DIALS (2.2.5) |
| Data scaling software | DIALS (2.2.5) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.450 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 140996 | 6703 |
| <I/σ(I)> | 4.75 | 0.25 |
| Completeness [%] | 99.1 | 95.5 |
| Redundancy | 13.5 | 12 |
| CC(1/2) | 0.990 | 0.281 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 303 | 100mM Tris pH 7.5 100mM NaCl 3% (v/v) Acetonitrile 5% (w/v)PEG 20k |
