8ORR
Outer membrane protein assembly factor BamB from Klebsiella pneumoniae
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-22 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.095, 50.095, 259.774 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.285 - 1.680 |
| Rwork | 0.171 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.426 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.285 | 1.610 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.073 | 0.665 |
| Number of reflections | 48078 | 3386 |
| <I/σ(I)> | 25.6 | 3.3 |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 24 | |
| CC(1/2) | 1.000 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22% PEG 400, 0.1 M sodium acetate trihydrate (pH 4.6) and 0.08 M cadmium acetate trihydrate |
