8ORN

Crystal structure of Xanthomonas campestris pv. campestris LolA-LolB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976254
Spacegroup name	H 3
Unit cell lengths	137.510, 137.510, 145.210
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.400 - 2.200
R-factor	0.2225
Rwork	0.221
R-free	0.26080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.015
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.19.2-4158)
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.400	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	51938	6459
<I/σ(I)>	10.6	1.3
Completeness [%]	99.9	99.8
Redundancy	2.8	2.3
CC(1/2)	0.998	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	50 mM Hepes pH 7.5, 2.5 M (NH4)2SO4 and 10% glycerol