8OR6

Crystal structure of Drosophila melanogaster alpha-amylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 1 21 1
Unit cell lengths	80.440, 73.090, 99.290
Unit cell angles	90.00, 98.27, 90.00

Refinement procedure

Resolution	44.780 - 2.500
R-factor	0.2106
Rwork	0.208
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.472
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.780	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	39454	4144
<I/σ(I)>	6.4	3
Completeness [%]	99.2
Redundancy	3.8	3.6
CC(1/2)	0.967	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20 % PEG MME 0.1 M NaCl 0.1 M bicine, pH 9.0