8OQJ
Peripheral subunit binding domain of the E. coli Dihydrolipoamide Acetyltransferase (E2) of the pyruvate dehydrogenase complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-05 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999874 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.576, 37.831, 37.834 |
| Unit cell angles | 100.39, 114.39, 101.18 |
Refinement procedure
| Resolution | 35.500 - 1.640 |
| R-factor | 0.2167 |
| Rwork | 0.215 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.781 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.064 | |
| Rmeas | 0.076 | |
| Rpim | 0.040 | 0.742 |
| Number of reflections | 18881 | 1783 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 96.3 | 90.46 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 100 mM NaOAc pH 5.0, 2 mM ZnCl2, 24 % (w/v) PEG 6000 |
