8OQG
Cross-linked crystal of dirhodium tetraacetate/ribonuclease A adduct in the P3221 space group (high temperature data collection)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2022-07-01 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.278, 67.278, 65.899 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.020 - 1.600 |
| Rwork | 0.182 |
| R-free | 0.20150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 3.219 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.020 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.079 | 1.062 |
| Rpim | 0.079 | 1.062 |
| Number of reflections | 23142 | 1111 |
| <I/σ(I)> | 6.9 | 0.6 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 1.8 | 1.8 |
| CC(1/2) | 0.994 | 0.339 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 2.5 M NaCl 3.3 M HCOONa 0.1 M Acetate buffer |
