8OQ2

Binding of NADP to a formate dehydrogenase from Starkeya novella.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-23
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.965
Spacegroup name	P 61
Unit cell lengths	132.265, 132.265, 302.952
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	114.600 - 1.910
Rwork	0.237
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	114.600	7.200
High resolution limit [Å]	1.910	1.910
Number of reflections	163262	3586
<I/σ(I)>	12
Completeness [%]	82.0	25.54
Redundancy	8
CC(1/2)	0.910	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	298	Citric acid pH 4.5, PEG 3350, magnesium chloride