8OQ1

Crystal structure of tailspike depolymerase (APK14_gp49) from Acinetobacter phage vB_AbaP_APK14

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	77.394, 77.394, 228.536
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	114.270 - 1.550
R-factor	0.20141
Rwork	0.200
R-free	0.23574
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	2.103
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	228.540	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.145	2.706
Rmeas	0.150	2.830
Rpim	0.039	0.819
Total number of observations	1713096	65962
Number of reflections	111761	5547
<I/σ(I)>	7.7	0.2
Completeness [%]	100.0
Redundancy	15.3	11.9
CC(1/2)	0.999	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	50mM CaCl2, 100mM Bis-tris pH 6.5, 30%PEG 550 MME